Note: For Precision Plus Protein Dual Xtra Prestained Standards the 2 kD band is not visible on fluorescent blots. Proteins were loaded along with the Precision Plus protein ladder (Bio-Rad, 1610376) in 415Mini-PROTEAN TGX Precast PAGE Gel (Bio-Rad. These standards can be detected by fluorescence when excited at red and green wavelengths. Blots were also loaded with Precision Plus Protein Western C standards (Bio-Rad, 1610376) Blots were dry transferred using the iBlot2 Dry Blotting System (Thermo Fisher, IB21001). Protein-Like Proteins of Ulva lactuca Are Plant Elicitors. In addition to the ability for total protein normalization (TPN) without the need. western blot assay and control for the presence of ulvan was done by TBO assay. Stain-Free technology adds speed and enables quality assessment at each step of a western blotting experiment from running gels to quantitating proteins. Julian received a Bachelor of Laws degree from City. Precision Plus Protein Dual Xtra Standards are a mixture of 12 recombinant proteins (2–250 kD), including nine blue-stained bands and three pink reference bands (2, 25, and 75 kD). Bio-Rad Stain-Free western blotting is a streamlined approach to getting better western blotting results. Previously, Paula was a Biology T eacher At Mayo High School at West.
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